ABSTRACT
Objective To explore the anti-HBV activity of anodonta polysaccharides (AP) and dose-effect relationship in vitro.Methods HepG2.2.15 cells were cultured in vitro and incubated at 37℃ for nine days with AP at a dilution ratio of 1∶10.The expression of HBsAg and HBeAg were detected using ELISA and HBV-DNA copies were detected by real-time fluorescent quantitative PCR.Based on Thiele-type continued-fraction interpolation method,the anti-HBV activity of AP was studied,and the IC50 and the maximum inhibition rate were calculated.Results AP had significant inhibitory effect on the expression of HBsAg and HBeAg in HepG2.2.15 cells in vitro,as well as HBV DNA replication.By Thiele-type continued-fraction interpolation the equations of dose-effect relationship were obtained to determine the maximum inhibition rates of AP on HBeAg and HBsAg secretion being 47.7％ and 56.4％,and the IC50 inhibiting the expression of HBeAg being 143.7mg/L.AP was also able to inhibit HBV-DNA replication and the maximum inhibition rate was 17.8％ with the same method above.Conclusion Anodonta polysaccharides have anti-HBV activity.The thiele-type continued-fraction interpolation method is simple and practical and could be used as a new method for the analysis of drug activity.
ABSTRACT
By analyzing the questionnaire survey of consciousness of rights protection and status quo of rights and interests among medical staff in three 3rd degree and A grade hospitals located in northwest Hubei province,this paper concludes that medical staff there have a generally strong consciousness of rights,while their rights and interests are not well protected by society and hospitals,especially certain aspects including personal security,occupational health,working hours,legal vacation,and the rights to be involved in hospital management.It is suggested that legal rights and interests of medical staff should be improved through legislation,social support,and the direction function of public opinions.